Facts-enriched characterization of mobile populations. Morphological information and facts from visuals can incorporate to your richness of flow cytometry data. For instance, the figure exhibits an or else traditional apoptosis assay making use of Annexin V and PI, adding cell imaging to characterize cells in Just about every inhabitants to expose morphologically distinctive attributes. These insights could not have been attained from multiplex staining by yourself.
Regardless of whether you’re a starter or an authority, examine our means to progress your exploration and abilities in flow cytometry.
Combine acoustic concentrating technology with superior-parameter Investigation for adaptable spectral unmixing and standard workflows.
Explore new insights into your experimental knowledge by visualizing populations employing mixed fluorescence and morphology facts. Our software package interface wizards help downsampling, concatenation (merged files) and dimensionality applications that are crafted in the application for an intuitive, streamlined info Examination working experience.
"A picture just isn't pretty worth one thousand dots, but it really genuinely helps you to see images. It is excellent to find out photographs that correlate with staining. By way of example, AnnexinV-optimistic cells which might be morphologically different than wholesome cells, or CD14+ monocytes which can be greater plus more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a highly skilled person has gated singlets confidently. After evaluating the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate contains more than 4% aggregates.
Pair your Attune flow cytometer having an autosampler for improved workflow performance, and insert robotic integration for max throughput.
Consistent picture excellent even at large throughput Acoustic concentrating as well as a superior-pace digicam Merge to picture these T cells persistently at low or significant flow prices. Easily regulate focus and digicam options to meet experimental prerequisites.
Early signs of this contamination had at first been dismissed as particles. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also enables you to use morphological features to discover interesting subpopulations that would not be evident from flow cytometry facts alone.
End users can check out occasion illustrations or photos in actual-time to substantiate sample good quality, improve protocols, and report the morphology of each and every cell populace recognized for analysis. Imaging capture also Advantages from acoustic concentrating, which really helps to situation the cells in order that a sharp, centered graphic is acquired.
Seem waves help specific mobile alignment, leading to Outstanding data top quality and ~10X speedier sample flow rates.
We click here previously shown the power of imaging Automobile-T/Ramos mobile interactions. Allow’s take a look at just the population of biggest fascination, the double good events, To find out more. We can easily now use prolonged morphological parameters (here, circularity and skewness of depth) to even more analyze the characteristics of these populations and refine gating on these gatherings, growing info robustness.
Constructed-in dimensionality reduction instruments amplified resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify envisioned populace resolution in lysed whole human blood. Person can watch activities from Just about every cluster for Visible confirmation of cell phenotypes.
Flexible payment program from Agilent Economic Options enable you to receive the crucial instrumentation you may need for fast undertaking, or to develop your lab.
At the heart of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technologies, which utilizes audio waves to meticulously align cells inside the flow mobile. This ground breaking approach surpasses conventional hydrodynamic strategies, causing more quickly processing speeds, Remarkable facts good quality, and improved sample throughput.
As the cell line experienced Formerly been analyzed over the Attune CytPix Flow Cytometer, the scientists went back to the images and have been in the position to document the microbial infection at least 5 days earlier.
Agilent Price Assure ensures you at the least ten years of instrument use within the day you buy. Usually, We are going to credit score you Along with the residual value of the program towards an upgraded model.
In this article, we demonstrate that by gating strategies working with graphic-based quantitative parameters can distinguish interacting cells from coincident events to additional properly assess interacting cells.